Second-generation transcriptome sequencing reveals the evolution of the giant horsetail
The giant horsetail is one of the oldest ferns. After undergoing a long and mysterious evolutionary process, the giant horsetail genome has a total of 107 chromosomes and still retains many characteristics of ancient creatures. However, research on the polyploid mechanism in the evolution of the giant horsetail is very limited, and the influence of the whole genome duplication event (WGD) on evolution is still under debate. This article analyzes the role of WGD in the evolution of the giant wooden thief by sequencing the transcriptome.
- Extracted RNA from the mixed tissue of the stem, leaf, and strobilus of the giant horsetail, and perform Illumina sequencing. A total of 220M reads (PE40)was obtained, and the Oases software was used for de novo transcriptome assembly;
- Construct the Ks age distribution of the giant horsetail to detect replication events;
- Perform functional enrichment analysis on homoeologous genes generated by genome-wide replication events.
- A total of 34,000 transcripts were assembled, with an average length of 1251bp.
- The giant horsetail has only experienced one polyploid event.
- The whole genome replication event enhances the adaptability of the giant horsetail.
Vanneste et al. (2015) Horsetails Are Ancient Polyploids: Evidence from Equisetum giganteum. Plant Cell.