PacBio also Updated at last! Both Software and Reagent, for Higher Throughput and Longer Reads!

Pacific Biosciences of California, Inc. formally announced a new version of Sequel® Software (V5.1) and a new polymerase on Mar. 7, 2018. Combined, these enhancements increase throughput and the overall performance of Single Molecule, Real-Time (SMRT®) Sequencing for key applications such as de novo assembly, structural variant detection, targeted sequencing, and RNA sequencing (Iso-Seq® method), making genomic research more economical.

With this release the Sequel System can achieve up to 10 Gb per SMRT Cell for genomic libraries, effectively doubling the throughput when using ultra-long inserts (>40 kb) for de novo genome assembly. For targeted and RNA sequencing, customers can achieve up to 20 Gb per SMRT Cell.

For human whole genome sequencing (WGS) studies, the new improvements support sensitive detection of structural variants with as little as 5- to 10-fold coverage per individual. As a result, customers can now complete low-cost WGS studies in thousands of individuals using fewer SMRT Cells.

For long amplicons (>3 kb), the new polymerase increases the number of high-quality sequences per SMRT Cell, reducing costs for HLA sequencing and other targeted applications. Further, software enhancements for multiplexed samples simplify the analytical workflow.

Appendix: Based on PacBio SMRT technology, NectOmics has assembled hundreds of genomes and authored mature process for full-length transcriptome, some published papers as follow:

Classic NextOmics genome-assembling projects

Classic NextOmics Full-length Transcriptome projects based on the Third Generation Sequencing Technology

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